TruSeq RNA Library Prep Kit v2

RNA sequencing Mouse - NSC-34

Experiment
RNA sequencing Mouse - NSC-34
Product
TruSeq RNA Library Prep Kit v2 from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Publication protocol

Three sets of NSC34 cells (transfected with wild-type or G93A mutant hSOD1) were grown and harvested with each set from a separate passage of single cell line. Following subcellular fractionation, transcriptomes of 12 samples were analysed by RNA-seq (Macrogen Inc. Seoul, Korea), as described previously. Briefly, 1 μg of the total RNA was analysed using the TruSeq RNA library kit to construct the cDNA libraries. The protocol included polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 100 nt paired-end sequencing using an Illumina HiSeq2000 (Illumina, San Diego, CA, USA). The libraries were quantified using quantitative real-time polymerase chain reaction (qPCR) according to the qPCR Quantification Protocol Guide. An Agilent Technologies 2100 Bioanalyzer was used for the qualification.

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Reviews

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Discussion

Discussion

5 years ago

Author: Panagiotis Karagiannis Greece

Using RNA from formalin fixed paraffin embedded tissue sections for sequencing

Hello there! I have some slides with mouse lung tumors and I would like to do rna sequencing. Will I be able to extract enough/good quality rna from RNA from formalin fixed paraffin embedded tissue sections for sequencing to be successful?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Mouse - NSC-34 using TruSeq RNA Library Prep Kit v2 from Illumina.

Paper title
Altered nucleocytoplasmic proteome and transcriptome distributions in an model of amyotrophic lateral sclerosis
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Manufacturer protocol

Download the product protocol from Illumina for TruSeq RNA Library Prep Kit v2 below.

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Videos

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